NAD+
For laboratory research use only — not for human or animal consumption.
Research-grade NAD+ (nicotinamide adenine dinucleotide), the central redox coenzyme of cellular energy metabolism, ≥99% HPLC purity with third-party batch-level Certificate of Analysis. For laboratory research use only.
Description
NAD+
NAD+ is a critical coenzyme central to cellular redox reactions and energy metabolism. Preclinical research explores its roles in mitochondrial function, sirtuin activation, and cellular aging processes.
Key Research Areas
- • Studied for its central role in cellular energy metabolism and maintenance of redox homeostasis.
- • Investigated for activation of sirtuin pathways and support of mitochondrial biogenesis and function in aging models.
- • Explored for protective effects against age-related metabolic decline and cellular damage in various research paradigms.
Product Specifications
| Product | NAD+ |
| CAS Number | 53-84-9 |
| Molecular Formula | C21H27N7O14P2 |
| Molar Mass | 663.4 g/mol |
| Sequence | N/A (dinucleotide coenzyme) |
| Synonyms | NAD+; Nicotinamide adenine dinucleotide; Coenzyme I |
| Purity | ≥99% (HPLC) |
| Form | Lyophilized powder |
| Storage | −20°C (−4°F) long-term; 2–8°C (36–46°F) reconstituted |
| Solubility | Bacteriostatic water for laboratory use |
Mechanism of Action
NAD+ (nicotinamide adenine dinucleotide) cycles between oxidized (NAD+) and reduced (NADH) forms as the principal electron carrier in glycolysis, the TCA cycle, and oxidative phosphorylation. Beyond redox chemistry it is a consumed substrate for sirtuin deacetylases (SIRT1–7), poly(ADP-ribose) polymerases (PARPs), and CD38, linking cellular NAD+ availability to chromatin regulation, DNA-repair signaling, and mitochondrial biogenesis. In aged rodent models tissue NAD+ declines and precursor supplementation has been reported to restore mitochondrial function markers. Observations summarized here derive from in vitro assays and animal models; the material sold on this page has not been evaluated in controlled human trials.
Research Literature
- • Covarrubias et al. (2021) — review of NAD+ metabolism and its roles in cellular processes during ageing. PMID: 33353981.
- • Imai & Guarente (2014) — NAD+ and sirtuins in aging and disease. PMID: 24786309.
- • Doke et al. (2023) — NAD+ precursor supplementation and mitochondrial-RNA-dependent inflammation in a mouse kidney-injury model. PMID: 36914909.
- • Additional peer-reviewed literature is indexed on PubMed under the compound name.
Reconstitution & Storage
Lyophilized NAD+ is stable at −20°C or colder in its sealed vial; −80°C is preferable for long-term archival. Reconstitute in bacteriostatic water (0.9% benzyl alcohol) to a laboratory working concentration of 10–50 mg/mL for in vitro use. Once reconstituted, refrigerate at 2–8°C and use within approximately 30 days. Aliquot into single-use vials at reconstitution to avoid repeated freeze-thaw cycles of the working solution. NAD+ solutions are light- and pH-sensitive; protect from light and keep buffered near neutral pH. Every batch ships with a third-party Certificate of Analysis; request the analytical file for your batch at /coa.
Research Use Only
For research use only. Not intended for human or veterinary consumption, therapeutic use, dietary supplementation, cosmetic use, or clinical application. Not FDA-approved. Purchasers confirm the material will be used solely for in vitro laboratory research and not administered to humans or animals.